miRNA: 3'- guUUCUuCCGCUACgaUAA--UGGG-GUG- -5'
miRNA: 3'- guuuCUUCCGCUACg---AUAAUGGGGuG- -5'
miRNA: 3'- guuuCUUCCGCUACGA------UAaUGGGgUG- -5'
miRNA: 3'- -GUUUCUUCCGCUACGAUAAUGGGGUG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32306 | 5' | -48.8 | NC_007024.1 | + | 32349 | 0.67 | 0.924589 |
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Target: 5'- -cGAGG-GGCGAUGgaGUUcgGCCCuCGCa -3' miRNA: 3'- guUUCUuCCGCUACgaUAA--UGGG-GUG- -5' |
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| 32306 | 5' | -48.8 | NC_007024.1 | + | 2367 | 0.71 | 0.778462 |
|
Target: 5'- ---cGGAGGUGAUGauggUGUUGCCCC-Cg -3' miRNA: 3'- guuuCUUCCGCUACg---AUAAUGGGGuG- -5' |
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| 32306 | 5' | -48.8 | NC_007024.1 | + | 45621 | 0.72 | 0.72364 |
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Target: 5'- ---cGGAGGCGGUGCUucggaaGUcGCCCgACg -3' miRNA: 3'- guuuCUUCCGCUACGA------UAaUGGGgUG- -5' |
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| 32306 | 5' | -48.8 | NC_007024.1 | + | 12889 | 1.1 | 0.00351 |
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Target: 5'- uCAAAGAAGGCGAUGCUAUUACCCCACu -3' miRNA: 3'- -GUUUCUUCCGCUACGAUAAUGGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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