Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32310 | 3' | -50.2 | NC_007024.1 | + | 48962 | 0.67 | 0.924782 |
Target: 5'- aCACGCacaGCUUUGGc--UGgGGCCGGGu -3' miRNA: 3'- gGUGCG---UGAAGCUauuACaCCGGUCC- -5' |
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32310 | 3' | -50.2 | NC_007024.1 | + | 1909 | 0.68 | 0.898601 |
Target: 5'- uCCACGUACUUUGcgauuGUAcgGUGGCa--- -3' miRNA: 3'- -GGUGCGUGAAGC-----UAUuaCACCGgucc -5' |
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32310 | 3' | -50.2 | NC_007024.1 | + | 38763 | 0.71 | 0.753701 |
Target: 5'- gCCACaGUACUggGAaGAUGUGGUCuGGg -3' miRNA: 3'- -GGUG-CGUGAagCUaUUACACCGGuCC- -5' |
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32310 | 3' | -50.2 | NC_007024.1 | + | 53575 | 0.71 | 0.732291 |
Target: 5'- -uGCGCGCUgaccguggCGGUAu--UGGCCGGGg -3' miRNA: 3'- ggUGCGUGAa-------GCUAUuacACCGGUCC- -5' |
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32310 | 3' | -50.2 | NC_007024.1 | + | 17845 | 1.13 | 0.002039 |
Target: 5'- aCCACGCACUUCGAUAAUGUGGCCAGGc -3' miRNA: 3'- -GGUGCGUGAAGCUAUUACACCGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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