Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32311 | 3' | -55.2 | NC_007024.1 | + | 4260 | 0.66 | 0.710063 |
Target: 5'- --cGUGGUGCAaaCCGCGuCGCCUaCCa -3' miRNA: 3'- guuUAUCGCGUg-GGCGCuGUGGA-GGc -5' |
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32311 | 3' | -55.2 | NC_007024.1 | + | 1573 | 0.66 | 0.699293 |
Target: 5'- ----aAGCGCgauGCCC-CGuCGCCUCCu -3' miRNA: 3'- guuuaUCGCG---UGGGcGCuGUGGAGGc -5' |
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32311 | 3' | -55.2 | NC_007024.1 | + | 20212 | 0.67 | 0.666637 |
Target: 5'- --cGUAGUGCuaaCCGCGAUACCUg-- -3' miRNA: 3'- guuUAUCGCGug-GGCGCUGUGGAggc -5' |
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32311 | 3' | -55.2 | NC_007024.1 | + | 17984 | 1.08 | 0.001314 |
Target: 5'- gCAAAUAGCGCACCCGCGACACCUCCGc -3' miRNA: 3'- -GUUUAUCGCGUGGGCGCUGUGGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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