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miRNA: 3'- cuauuaUGGAAC-CGUAGaa---CAGGCAG- -5'
miRNA: 3'- cuauuaUGGAACC-GUAGAACaGGCaG- -5'
miRNA: 3'- -CUAUUAUGGAACCGUAGAACAGGCAG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 32316 | 3' | -47 | NC_007024.1 | + | 25891 | 0.67 | 0.971163 |
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Target: 5'- -----cGCCUUGaGUAUCaguaaGUCCGUCg -3' miRNA: 3'- cuauuaUGGAAC-CGUAGaa---CAGGCAG- -5' |
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| 32316 | 3' | -47 | NC_007024.1 | + | 16356 | 0.69 | 0.946357 |
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Target: 5'- -----aGCCUUGGcCGUCUUG-CCGcCa -3' miRNA: 3'- cuauuaUGGAACC-GUAGAACaGGCaG- -5' |
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| 32316 | 3' | -47 | NC_007024.1 | + | 25642 | 1.11 | 0.004937 |
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Target: 5'- aGAUAAUACCUUGGCAUCUUGUCCGUCu -3' miRNA: 3'- -CUAUUAUGGAACCGUAGAACAGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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