miRNA: 3'- -GGACUgga----UACUAAUGUGGU---UAGGCU- -5'
miRNA: 3'- ggACUGGauaCUAa---UGUGGUUAGGCU- -5'
miRNA: 3'- -GGACUGGAUACUAAUGUGGUUAGGCU- -5'
miRNA: 3'- -GGACUGGauacuaaUGUGGUU-AGGCU- -5'
miRNA: 3'- -GGACUGG-AUACUa-AUGUGGu----UAGGCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32319 | 5' | -46.8 | NC_007024.1 | + | 11999 | 0.72 | 0.859108 |
|
Target: 5'- aCCUGGagaggugGUGGUUGCACCAacgAUCUGAg -3' miRNA: 3'- -GGACUgga----UACUAAUGUGGU---UAGGCU- -5' |
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| 32319 | 5' | -46.8 | NC_007024.1 | + | 18779 | 0.73 | 0.831839 |
|
Target: 5'- -gUGGCCcg-GAUcuugACGCCGAUCCGAg -3' miRNA: 3'- ggACUGGauaCUAa---UGUGGUUAGGCU- -5' |
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| 32319 | 5' | -46.8 | NC_007024.1 | + | 30985 | 1.14 | 0.004442 |
|
Target: 5'- aCCUGACCUAUGAUUACACCAAUCCGAa -3' miRNA: 3'- -GGACUGGAUACUAAUGUGGUUAGGCU- -5' |
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| 32319 | 5' | -46.8 | NC_007024.1 | + | 34307 | 0.67 | 0.981636 |
|
Target: 5'- uCUUGACCaauucggGCACCAGcUCCGAu -3' miRNA: 3'- -GGACUGGauacuaaUGUGGUU-AGGCU- -5' |
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| 32319 | 5' | -46.8 | NC_007024.1 | + | 52464 | 0.7 | 0.94782 |
|
Target: 5'- gCCUGACCaUGUGGcgUAUACUuaccuGUCUGAc -3' miRNA: 3'- -GGACUGG-AUACUa-AUGUGGu----UAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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