miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32330 5' -48.6 NC_007024.1 + 685 0.66 0.963601
Target:  5'- gAGgAAGaUGCgcgUAAGGUCAUCAUCg- -3'
miRNA:   3'- -UCgUUC-AUGaa-GUUCCGGUAGUAGag -5'
32330 5' -48.6 NC_007024.1 + 53888 0.66 0.959608
Target:  5'- uAGCGAGUGugccCUggAGGGCCugaAUCUCu -3'
miRNA:   3'- -UCGUUCAU----GAagUUCCGGuagUAGAG- -5'
32330 5' -48.6 NC_007024.1 + 8537 0.67 0.923194
Target:  5'- aGGCcAGUGCguugcccucgauUUCGgugaAGGgCAUCAUCUCg -3'
miRNA:   3'- -UCGuUCAUG------------AAGU----UCCgGUAGUAGAG- -5'
32330 5' -48.6 NC_007024.1 + 47302 1.12 0.002595
Target:  5'- gAGCAAGUACUUCAAGGCCAUCAUCUCg -3'
miRNA:   3'- -UCGUUCAUGAAGUUCCGGUAGUAGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.