miRNA display CGI

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ID	Location	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
32331	3'	-51.6	NC_007024.1	+	26577	0.66	0.873893
	Target:  5'- -cGCCC--AUUCCgugGCGCUuuuacaacAACCGCu -3' miRNA:   3'- cuCGGGaaUAGGGaa-UGCGA--------UUGGCG- -5'
32331	3'	-51.6	NC_007024.1	+	53601	0.66	0.865707
	Target:  5'- aGAcCCaCUUAUCCaugaacaucgCUUgcGCGCUGACCGUg -3' miRNA:   3'- -CUcGG-GAAUAGG----------GAA--UGCGAUUGGCG- -5'
32331	3'	-51.6	NC_007024.1	+	11047	0.67	0.830463
	Target:  5'- cGAGCCCUUcUCCaacugcUGCGCauaUGGCgCGCc -3' miRNA:   3'- -CUCGGGAAuAGGga----AUGCG---AUUG-GCG- -5'
32331	3'	-51.6	NC_007024.1	+	14291	0.68	0.791682
	Target:  5'- aAGCCCUUugcccagCCCUUGgGCUGcGCCa- -3' miRNA:   3'- cUCGGGAAua-----GGGAAUgCGAU-UGGcg -5'
32331	3'	-51.6	NC_007024.1	+	23278	0.68	0.771185
	Target:  5'- gGAGCCaUUUGUCUggaagUACGUgGACCGCa -3' miRNA:   3'- -CUCGG-GAAUAGGga---AUGCGaUUGGCG- -5'
32331	3'	-51.6	NC_007024.1	+	6241	0.69	0.706436
	Target:  5'- -cGCCCUUuUUCUUgacuucGCGCUGACCGa -3' miRNA:   3'- cuCGGGAAuAGGGAa-----UGCGAUUGGCg -5'
32331	3'	-51.6	NC_007024.1	+	55267	1.12	0.001427
	Target:  5'- gGAGCCCUUAUCCCUUACGCUAACCGCu -3' miRNA:   3'- -CUCGGGAAUAGGGAAUGCGAUUGGCG- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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