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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32335 | 3' | -56.3 | NC_007024.1 | + | 52579 | 0.66 | 0.624719 |
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Target: 5'- aGUGGAugcUUGaCUugaGGCCGGCCGAGa- -3' miRNA: 3'- aUACCUu--AACcGA---CCGGUCGGCUCcg -5' |
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| 32335 | 3' | -56.3 | NC_007024.1 | + | 14843 | 0.66 | 0.6136 |
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Target: 5'- aGUGGAAgUGGCguUGGCCcGCUuuuuGGCc -3' miRNA: 3'- aUACCUUaACCG--ACCGGuCGGcu--CCG- -5' |
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| 32335 | 3' | -56.3 | NC_007024.1 | + | 22517 | 0.66 | 0.602497 |
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Target: 5'- -cUGcGAAuUUGGCaGGCCAGCCu-GGUu -3' miRNA: 3'- auAC-CUU-AACCGaCCGGUCGGcuCCG- -5' |
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| 32335 | 3' | -56.3 | NC_007024.1 | + | 54845 | 0.68 | 0.504755 |
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Target: 5'- gGUGGuggUGGaCUGGCUgccgaagcgGGCCaGGGCg -3' miRNA: 3'- aUACCuuaACC-GACCGG---------UCGGcUCCG- -5' |
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| 32335 | 3' | -56.3 | NC_007024.1 | + | 43675 | 0.71 | 0.343092 |
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Target: 5'- cGUGGAAgacaucugcaUGGCUucGGCCGGCCGcugugcucGGGUg -3' miRNA: 3'- aUACCUUa---------ACCGA--CCGGUCGGC--------UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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