Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32344 | 3' | -49.5 | NC_007024.1 | + | 19606 | 0.66 | 0.939436 |
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Target: 5'- -aCGCCACUCGGuGGUggaCGGAccauAUCAUc -3' miRNA: 3'- cgGCGGUGAGUU-CCAaa-GUCU----UAGUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 21448 | 0.68 | 0.8948 |
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Target: 5'- uCCaCCACUCGccaGGGUUguUCGGAuuuUCACg -3' miRNA: 3'- cGGcGGUGAGU---UCCAA--AGUCUu--AGUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 24045 | 0.68 | 0.879382 |
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Target: 5'- cGCgGCCAUaaaUCGgcAGGUcUCAcGGAUCGCa -3' miRNA: 3'- -CGgCGGUG---AGU--UCCAaAGU-CUUAGUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 43647 | 0.7 | 0.797183 |
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Target: 5'- gGCCGCUguGCUC-GGGUgUCGuaccuGAAUCACg -3' miRNA: 3'- -CGGCGG--UGAGuUCCAaAGU-----CUUAGUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 42477 | 0.7 | 0.776547 |
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Target: 5'- cGCCGCUACaUCAAGGaacUCAuuaaGGUCACa -3' miRNA: 3'- -CGGCGGUG-AGUUCCaa-AGUc---UUAGUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 18466 | 0.71 | 0.765977 |
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Target: 5'- gGCCGCCcaagauauACUCGAGGUUgaUCAuGAcacgCACa -3' miRNA: 3'- -CGGCGG--------UGAGUUCCAA--AGU-CUua--GUG- -5' |
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| 32344 | 3' | -49.5 | NC_007024.1 | + | 47720 | 0.71 | 0.755256 |
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Target: 5'- gGCgGCCAUgUCGAGGUcUCGGAcgaAUCAUc -3' miRNA: 3'- -CGgCGGUG-AGUUCCAaAGUCU---UAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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