Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32348 | 5' | -53.7 | NC_007024.1 | + | 9188 | 0.66 | 0.771185 |
Target: 5'- cCGGCCGAACCgcGGGCcg-UCagGCCGGc -3' miRNA: 3'- uGUCGGUUUGG--UCCGauaAG--CGGCUa -5' |
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32348 | 5' | -53.7 | NC_007024.1 | + | 3129 | 0.67 | 0.717494 |
Target: 5'- aACuGCCuuGCCGGGCaGUcggCGCCGu- -3' miRNA: 3'- -UGuCGGuuUGGUCCGaUAa--GCGGCua -5' |
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32348 | 5' | -53.7 | NC_007024.1 | + | 33636 | 0.67 | 0.684101 |
Target: 5'- aGCAGCCGAugaAGGCcccuggCGCCGAc -3' miRNA: 3'- -UGUCGGUUuggUCCGauaa--GCGGCUa -5' |
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32348 | 5' | -53.7 | NC_007024.1 | + | 34153 | 0.68 | 0.638892 |
Target: 5'- gGCAGUCGAGCCGGGUgcgg-GCCuGAUg -3' miRNA: 3'- -UGUCGGUUUGGUCCGauaagCGG-CUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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