miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32354 3' -52.1 NC_007024.1 + 52380 0.72 0.499212
Target:  5'- gGUGUAuCCUUGGGCaUCgCACAGuGGUg -3'
miRNA:   3'- aCAUAU-GGAACCUG-AG-GUGUCuCCGa -5'
32354 3' -52.1 NC_007024.1 + 33892 0.66 0.861704
Target:  5'- aGUGUuuGCCUUGGGCaUCC-CGGuuaccucguugaAGGCg -3'
miRNA:   3'- aCAUA--UGGAACCUG-AGGuGUC------------UCCGa -5'
32354 3' -52.1 NC_007024.1 + 26476 0.67 0.806685
Target:  5'- --aAUGCCUUGGACgacgCCAUuuaccAGGCg -3'
miRNA:   3'- acaUAUGGAACCUGa---GGUGuc---UCCGa -5'
32354 3' -52.1 NC_007024.1 + 8734 0.68 0.733839
Target:  5'- ----cGCCUUGGACgcgaccugCACAGAGGUc -3'
miRNA:   3'- acauaUGGAACCUGag------GUGUCUCCGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.