Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32355 | 3' | -53 | NC_007024.1 | + | 15947 | 0.66 | 0.815978 |
Target: 5'- cUUGGACCCau-GAACG-CGCCCAu- -3' miRNA: 3'- uAACUUGGGgucUUUGCuGCGGGUcu -5' |
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32355 | 3' | -53 | NC_007024.1 | + | 22217 | 0.66 | 0.786481 |
Target: 5'- cUUGAugCUCGGc-AUGGCGCCCAa- -3' miRNA: 3'- uAACUugGGGUCuuUGCUGCGGGUcu -5' |
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32355 | 3' | -53 | NC_007024.1 | + | 23256 | 0.66 | 0.796498 |
Target: 5'- -gUGGACCgCAGGAACGGuucaggauguUGgCCAGAc -3' miRNA: 3'- uaACUUGGgGUCUUUGCU----------GCgGGUCU- -5' |
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32355 | 3' | -53 | NC_007024.1 | + | 29961 | 0.73 | 0.400915 |
Target: 5'- --gGAcCCCCAGGAACGuuGCCCAu- -3' miRNA: 3'- uaaCUuGGGGUCUUUGCugCGGGUcu -5' |
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32355 | 3' | -53 | NC_007024.1 | + | 43383 | 0.76 | 0.281486 |
Target: 5'- aGUUGGACUCCGucgcccggccaggcGAuauCGACGCCCAGAa -3' miRNA: 3'- -UAACUUGGGGU--------------CUuu-GCUGCGGGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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