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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32355 | 5' | -55.3 | NC_007024.1 | + | 6505 | 0.66 | 0.750592 |
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Target: 5'- ---gUGGGCGaUCGAUGaacaCGCCaacgcGGCCc -3' miRNA: 3'- gaagACCCGC-AGCUAUa---GCGGa----CCGG- -5' |
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| 32355 | 5' | -55.3 | NC_007024.1 | + | 54746 | 0.66 | 0.697737 |
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Target: 5'- -gUCUGGGU---GGUAUCGgUCUGGUCg -3' miRNA: 3'- gaAGACCCGcagCUAUAGC-GGACCGG- -5' |
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| 32355 | 5' | -55.3 | NC_007024.1 | + | 7792 | 0.68 | 0.588594 |
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Target: 5'- -gUCUGGGUG-CGGUgAUUGaCCaGGCCu -3' miRNA: 3'- gaAGACCCGCaGCUA-UAGC-GGaCCGG- -5' |
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| 32355 | 5' | -55.3 | NC_007024.1 | + | 23143 | 0.68 | 0.577726 |
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Target: 5'- --aCUGaagccaGGCGUUGGaGUUGCCaUGGCCa -3' miRNA: 3'- gaaGAC------CCGCAGCUaUAGCGG-ACCGG- -5' |
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| 32355 | 5' | -55.3 | NC_007024.1 | + | 50357 | 0.69 | 0.556132 |
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Target: 5'- gUUCUGGGCGgCGGUggCGaCgCUGGUg -3' miRNA: 3'- gAAGACCCGCaGCUAuaGC-G-GACCGg -5' |
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| 32355 | 5' | -55.3 | NC_007024.1 | + | 37902 | 0.71 | 0.404258 |
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Target: 5'- -cUCUcGGGCG-CGAUcgCGCUcaaccugUGGCCg -3' miRNA: 3'- gaAGA-CCCGCaGCUAuaGCGG-------ACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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