miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32359 3' -56.8 NC_007024.1 + 43661 0.66 0.658515
Target:  5'- -gCAUGGCuuCGGcCGGCCGCUgugcUCGGGu -3'
miRNA:   3'- ugGUGUCGu-GCUaGCCGGCGA----AGCUC- -5'
32359 3' -56.8 NC_007024.1 + 13831 0.66 0.62584
Target:  5'- uGCCuCgAGCAUGcagaaccaCGGCCGCUUCaGAGa -3'
miRNA:   3'- -UGGuG-UCGUGCua------GCCGGCGAAG-CUC- -5'
32359 3' -56.8 NC_007024.1 + 46365 0.67 0.582391
Target:  5'- cCCGCAGaACGAUUuGCCGCU-CGAu -3'
miRNA:   3'- uGGUGUCgUGCUAGcCGGCGAaGCUc -5'
32359 3' -56.8 NC_007024.1 + 10957 0.68 0.497893
Target:  5'- uGCCGgAGUugGAUUGGCCGgaUUGc- -3'
miRNA:   3'- -UGGUgUCGugCUAGCCGGCgaAGCuc -5'
32359 3' -56.8 NC_007024.1 + 34731 0.7 0.382956
Target:  5'- -aCGCAGUAUcgGGUCGGCaGCUUCGAc -3'
miRNA:   3'- ugGUGUCGUG--CUAGCCGgCGAAGCUc -5'
32359 3' -56.8 NC_007024.1 + 9056 0.71 0.365575
Target:  5'- aACCACGGCACGccgcgguagGUCGGgauuugacgaCCGCU-CGGGa -3'
miRNA:   3'- -UGGUGUCGUGC---------UAGCC----------GGCGAaGCUC- -5'
32359 3' -56.8 NC_007024.1 + 30071 0.73 0.279984
Target:  5'- cAUgAUGGCgaugGCGAUgcUGGCCGCUUCGAGg -3'
miRNA:   3'- -UGgUGUCG----UGCUA--GCCGGCGAAGCUC- -5'
32359 3' -56.8 NC_007024.1 + 26896 0.73 0.27236
Target:  5'- cACCGCAGCAauucucuCGAUCGGCgCGUgcaaagUCGAu -3'
miRNA:   3'- -UGGUGUCGU-------GCUAGCCG-GCGa-----AGCUc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.