Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32359 | 3' | -56.8 | NC_007024.1 | + | 43661 | 0.66 | 0.658515 |
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Target: 5'- -gCAUGGCuuCGGcCGGCCGCUgugcUCGGGu -3' miRNA: 3'- ugGUGUCGu-GCUaGCCGGCGA----AGCUC- -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 13831 | 0.66 | 0.62584 |
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Target: 5'- uGCCuCgAGCAUGcagaaccaCGGCCGCUUCaGAGa -3' miRNA: 3'- -UGGuG-UCGUGCua------GCCGGCGAAG-CUC- -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 46365 | 0.67 | 0.582391 |
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Target: 5'- cCCGCAGaACGAUUuGCCGCU-CGAu -3' miRNA: 3'- uGGUGUCgUGCUAGcCGGCGAaGCUc -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 10957 | 0.68 | 0.497893 |
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Target: 5'- uGCCGgAGUugGAUUGGCCGgaUUGc- -3' miRNA: 3'- -UGGUgUCGugCUAGCCGGCgaAGCuc -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 34731 | 0.7 | 0.382956 |
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Target: 5'- -aCGCAGUAUcgGGUCGGCaGCUUCGAc -3' miRNA: 3'- ugGUGUCGUG--CUAGCCGgCGAAGCUc -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 9056 | 0.71 | 0.365575 |
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Target: 5'- aACCACGGCACGccgcgguagGUCGGgauuugacgaCCGCU-CGGGa -3' miRNA: 3'- -UGGUGUCGUGC---------UAGCC----------GGCGAaGCUC- -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 30071 | 0.73 | 0.279984 |
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Target: 5'- cAUgAUGGCgaugGCGAUgcUGGCCGCUUCGAGg -3' miRNA: 3'- -UGgUGUCG----UGCUA--GCCGGCGAAGCUC- -5' |
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| 32359 | 3' | -56.8 | NC_007024.1 | + | 26896 | 0.73 | 0.27236 |
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Target: 5'- cACCGCAGCAauucucuCGAUCGGCgCGUgcaaagUCGAu -3' miRNA: 3'- -UGGUGUCGU-------GCUAGCCG-GCGa-----AGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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