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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32362 | 3' | -59.7 | NC_007024.1 | + | 9700 | 0.72 | 0.182075 |
Target: 5'- gGUGCCGGUCaCAGaggGGUCGCC--GGCa -3' miRNA: 3'- -UACGGCUAGcGUCa--CCAGCGGgaCCG- -5' |
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32362 | 3' | -59.7 | NC_007024.1 | + | 48300 | 0.7 | 0.274701 |
Target: 5'- uUGaUCGAUCGCGgcGUGaGUCcucaaGCCCUGGCc -3' miRNA: 3'- uAC-GGCUAGCGU--CAC-CAG-----CGGGACCG- -5' |
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32362 | 3' | -59.7 | NC_007024.1 | + | 54886 | 0.67 | 0.392503 |
Target: 5'- uUGCCGA---CGGUGGU-GCCCagGGCa -3' miRNA: 3'- uACGGCUagcGUCACCAgCGGGa-CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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