miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3237 5' -53.4 NC_001514.1 + 6099 0.66 0.260787
Target:  5'- aAGGGCcCUGAcccuguccUCAUauggGGGCGAGGCucagccUGCa -3'
miRNA:   3'- -UCCUG-GACU--------AGUG----CCCGUUCUGu-----ACG- -5'
3237 5' -53.4 NC_001514.1 + 2457 0.68 0.204879
Target:  5'- cGGGCCUGGgcuccaggaguugcgCGgGGGCAAGAUuuuggaggGCa -3'
miRNA:   3'- uCCUGGACUa--------------GUgCCCGUUCUGua------CG- -5'
3237 5' -53.4 NC_001514.1 + 186 0.8 0.023283
Target:  5'- aAGGACCUGAUCACGcaCAGaACAUGCa -3'
miRNA:   3'- -UCCUGGACUAGUGCccGUUcUGUACG- -5'
3237 5' -53.4 NC_001514.1 + 8507 0.8 0.023283
Target:  5'- aAGGACCUGAUCACGcaCAGaACAUGCa -3'
miRNA:   3'- -UCCUGGACUAGUGCccGUUcUGUACG- -5'
3237 5' -53.4 NC_001514.1 + 100 1.11 6.9e-05
Target:  5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3'
miRNA:   3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5'
3237 5' -53.4 NC_001514.1 + 143 1.11 6.9e-05
Target:  5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3'
miRNA:   3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5'
3237 5' -53.4 NC_001514.1 + 8421 1.11 6.9e-05
Target:  5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3'
miRNA:   3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5'
3237 5' -53.4 NC_001514.1 + 8464 1.11 6.9e-05
Target:  5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3'
miRNA:   3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.