Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3237 | 5' | -53.4 | NC_001514.1 | + | 6099 | 0.66 | 0.260787 |
Target: 5'- aAGGGCcCUGAcccuguccUCAUauggGGGCGAGGCucagccUGCa -3' miRNA: 3'- -UCCUG-GACU--------AGUG----CCCGUUCUGu-----ACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 2457 | 0.68 | 0.204879 |
Target: 5'- cGGGCCUGGgcuccaggaguugcgCGgGGGCAAGAUuuuggaggGCa -3' miRNA: 3'- uCCUGGACUa--------------GUgCCCGUUCUGua------CG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 186 | 0.8 | 0.023283 |
Target: 5'- aAGGACCUGAUCACGcaCAGaACAUGCa -3' miRNA: 3'- -UCCUGGACUAGUGCccGUUcUGUACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 8507 | 0.8 | 0.023283 |
Target: 5'- aAGGACCUGAUCACGcaCAGaACAUGCa -3' miRNA: 3'- -UCCUGGACUAGUGCccGUUcUGUACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 100 | 1.11 | 6.9e-05 |
Target: 5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3' miRNA: 3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 143 | 1.11 | 6.9e-05 |
Target: 5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3' miRNA: 3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 8421 | 1.11 | 6.9e-05 |
Target: 5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3' miRNA: 3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5' |
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3237 | 5' | -53.4 | NC_001514.1 | + | 8464 | 1.11 | 6.9e-05 |
Target: 5'- gAGGACCUGAUCACGGGCAAGACAUGCc -3' miRNA: 3'- -UCCUGGACUAGUGCCCGUUCUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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