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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32374 | 3' | -48.6 | NC_007038.1 | + | 2363 | 0.69 | 0.450373 |
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Target: 5'- ---uUAUGCaCCCUUUUACCUUUCAGu -3' miRNA: 3'- uucuGUAUGcGGGAAAGUGGAAAGUU- -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 2519 | 0.71 | 0.373027 |
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Target: 5'- ---uUAUACGCCCUUUCACCa----- -3' miRNA: 3'- uucuGUAUGCGGGAAAGUGGaaaguu -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 2422 | 0.72 | 0.296337 |
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Target: 5'- -uGAgAUACGCCCUUUCAgCUUUa-- -3' miRNA: 3'- uuCUgUAUGCGGGAAAGUgGAAAguu -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 1980 | 0.74 | 0.232473 |
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Target: 5'- ---uUAUGCGCCCUUUUACCUUgCAAa -3' miRNA: 3'- uucuGUAUGCGGGAAAGUGGAAaGUU- -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 2036 | 0.77 | 0.139247 |
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Target: 5'- --aAUAUGCGCCCUUUUACCUUUgCAAa -3' miRNA: 3'- uucUGUAUGCGGGAAAGUGGAAA-GUU- -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 2110 | 0.99 | 0.003357 |
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Target: 5'- uAAGACAUACGCCCUUUUACCUUUCAAu -3' miRNA: 3'- -UUCUGUAUGCGGGAAAGUGGAAAGUU- -5' |
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| 32374 | 3' | -48.6 | NC_007038.1 | + | 2437 | 1.04 | 0.001384 |
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Target: 5'- cAAGACAUACGCCCUUUCACCUUUCAAa -3' miRNA: 3'- -UUCUGUAUGCGGGAAAGUGGAAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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