miRNA display CGI

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ID	Location	Perfect MFE.*	RefSeq Acc.	strand	Start Position	R_P_ratio#	P value
	Predicted miRNA align pattern
32383	5'	-49	NC_007041.1	+	5267	0.66	0.597637
	Target:  5'- cAACAgCGgaaaGCCAGCGCUAGGUuGCCc- -3' miRNA:   3'- -UUGUaGC----CGGUUGUGAUCUAuUGGau -5'
32383	5'	-49	NC_007041.1	+	4901	0.69	0.391409
	Target:  5'- cGGC-UUGGCCAAgGCUAGGUuAUCUAg -3' miRNA:   3'- -UUGuAGCCGGUUgUGAUCUAuUGGAU- -5'
32383	5'	-49	NC_007041.1	+	5673	0.7	0.359967
	Target:  5'- uAGCuagGGCCGACACUGGGaAGCCa- -3' miRNA:   3'- -UUGuagCCGGUUGUGAUCUaUUGGau -5'
32383	5'	-49	NC_007041.1	+	5741	0.7	0.349886
	Target:  5'- aAGCA-CGGaCCAGgACUGGGUAACCc- -3' miRNA:   3'- -UUGUaGCC-GGUUgUGAUCUAUUGGau -5'
32383	5'	-49	NC_007041.1	+	4954	1.05	0.000969
	Target:  5'- uAACAUCGGCCAACACUAGAUAACCUAg -3' miRNA:   3'- -UUGUAGCCGGUUGUGAUCUAUUGGAU- -5'

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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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