Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32384 | 3' | -53.2 | NC_007041.1 | + | 5641 | 1.06 | 0.000285 |
Target: 5'- cCUUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- -GAAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5589 | 1.06 | 0.000285 |
Target: 5'- cCUUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- -GAAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5485 | 1.06 | 0.000285 |
Target: 5'- cCUUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- -GAAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5381 | 1.06 | 0.000285 |
Target: 5'- cCUUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- -GAAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5535 | 1.02 | 0.00065 |
Target: 5'- -cUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- gaAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5431 | 1.02 | 0.00065 |
Target: 5'- -cUAGCUUAGGUCCAGUCGUGGGCAAc -3' miRNA: 3'- gaAUCGAAUCCAGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5327 | 0.86 | 0.010504 |
Target: 5'- -cUAGCUaAGGcCCAGUCGUGGGCAAc -3' miRNA: 3'- gaAUCGAaUCCaGGUCAGCACCCGUU- -5' |
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32384 | 3' | -53.2 | NC_007041.1 | + | 5691 | 0.66 | 0.352403 |
Target: 5'- -cUAGCUaAGGcCCAGgacUGGGCAAc -3' miRNA: 3'- gaAUCGAaUCCaGGUCagcACCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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