Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32384 | 5' | -54.4 | NC_007041.1 | + | 5295 | 0.67 | 0.255524 |
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Target: 5'- uGGCUUUCCGcUGUUGGCCCc------ -3' miRNA: 3'- -CCGAAGGGUuACAGCCGGGaucgauu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5652 | 0.68 | 0.207459 |
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Target: 5'- aGGUUgCCCAGUccUgGGCCUUAGCUAGg -3' miRNA: 3'- -CCGAaGGGUUAc-AgCCGGGAUCGAUU- -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5658 | 0.89 | 0.005483 |
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Target: 5'- uGGCUUCCCAAUGUCaGCCUUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGcCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5606 | 0.89 | 0.005483 |
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Target: 5'- uGGCUUCCCAAUGUCaGCCUUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGcCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5502 | 0.89 | 0.005483 |
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Target: 5'- uGGCUUCCCAAUGUCaGCCUUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGcCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5398 | 0.89 | 0.005483 |
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Target: 5'- uGGCUUCCCAAUGUCaGCCUUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGcCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5554 | 1.03 | 0.000361 |
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Target: 5'- uGGCUUCCCAAUGUCGGCCCUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGCCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5450 | 1.03 | 0.000361 |
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Target: 5'- uGGCUUCCCAAUGUCGGCCCUAGCUu- -3' miRNA: 3'- -CCGAAGGGUUACAGCCGGGAUCGAuu -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5710 | 1.08 | 0.000144 |
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Target: 5'- uGGCUUCCCAGUGUCGGCCCUAGCUAAg -3' miRNA: 3'- -CCGAAGGGUUACAGCCGGGAUCGAUU- -5' |
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| 32384 | 5' | -54.4 | NC_007041.1 | + | 5346 | 1.08 | 0.000144 |
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Target: 5'- uGGCUUCCCAAUGUCGGCCCUAGCUAAg -3' miRNA: 3'- -CCGAAGGGUUACAGCCGGGAUCGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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