Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32388 | 3' | -54.5 | NC_007041.1 | + | 5304 | 1.08 | 0.000144 |
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Target: 5'- gGCCUUAGCUAGGGCCGACAUUGGGAAg -3' miRNA: 3'- -CGGAAUCGAUCCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5668 | 1.01 | 0.000507 |
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Target: 5'- gGCCUUAGCUAGGGCCGACAcUGGGAAg -3' miRNA: 3'- -CGGAAUCGAUCCCGGCUGUaACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5409 | 0.96 | 0.001271 |
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Target: 5'- aCCUaAGCUAGGGCCGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUCCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5513 | 0.96 | 0.001271 |
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Target: 5'- aCCUaAGCUAGGGCCGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUCCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5357 | 0.8 | 0.024286 |
|
Target: 5'- aCCUaAGCUAaGGCUGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUcCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5461 | 0.8 | 0.024286 |
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Target: 5'- aCCUaAGCUAaGGCUGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUcCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5565 | 0.8 | 0.024286 |
|
Target: 5'- aCCUaAGCUAaGGCUGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUcCCGGCUGUAACCCUU- -5' |
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| 32388 | 3' | -54.5 | NC_007041.1 | + | 5617 | 0.8 | 0.024286 |
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Target: 5'- aCCUaAGCUAaGGCUGACAUUGGGAAg -3' miRNA: 3'- cGGAaUCGAUcCCGGCUGUAACCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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