miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32394 5' -58.3 NC_007050.1 + 11393 0.66 0.437216
Target:  5'- aAGUUAGCuauccaaCCaGUUAUUGCUACUGCAa -3'
miRNA:   3'- -UCGGUCGc------GGcCGAUAACGAUGGCGU- -5'
32394 5' -58.3 NC_007050.1 + 10499 0.73 0.152878
Target:  5'- uAGCCGGCGCUGGCU-UUGUagcauuUGgCGCAa -3'
miRNA:   3'- -UCGGUCGCGGCCGAuAACG------AUgGCGU- -5'
32394 5' -58.3 NC_007050.1 + 10557 1.08 0.000364
Target:  5'- aAGCCAGCGCCGGCUAUUGCUACCGCAc -3'
miRNA:   3'- -UCGGUCGCGGCCGAUAACGAUGGCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.