miRNA: 3'- -GUUUUCCG--------------UGCGu-------UCCCUGCugUGuu -5'
miRNA: 3'- guUUUCCGUGCg---UUCCcUGCUGUGUU- -5'
miRNA: 3'- -GUUUUCCgUGCGUUCCCUGCUGU-GUu -5'
miRNA: 3'- guuUUCCGUGCGUUCCCUGCuGUGUu -5'
miRNA: 3'- -GUUUUCCGUGCGUUCCCUGCUGUGUU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32396 | 5' | -51.8 | NC_007051.1 | + | 13011 | 0.69 | 0.59843 |
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Target: 5'- gCAGAAGGCgcagguauguauccGCGCuuuagcguAGGGACGugGCu- -3' miRNA: 3'- -GUUUUCCG--------------UGCGu-------UCCCUGCugUGuu -5' |
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| 32396 | 5' | -51.8 | NC_007051.1 | + | 38347 | 0.71 | 0.466675 |
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Target: 5'- -uAAAGGCACGguuaAAGGcACGGCACAAa -3' miRNA: 3'- guUUUCCGUGCg---UUCCcUGCUGUGUU- -5' |
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| 32396 | 5' | -51.8 | NC_007051.1 | + | 38465 | 0.83 | 0.091597 |
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Target: 5'- aUAAAAGGgACGCAAGGGACGAUAgCAc -3' miRNA: 3'- -GUUUUCCgUGCGUUCCCUGCUGU-GUu -5' |
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| 32396 | 5' | -51.8 | NC_007051.1 | + | 38515 | 0.88 | 0.042414 |
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Target: 5'- --uAAGGCACGCAAGGGACG-CACAu -3' miRNA: 3'- guuUUCCGUGCGUUCCCUGCuGUGUu -5' |
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| 32396 | 5' | -51.8 | NC_007051.1 | + | 38378 | 1.06 | 0.002171 |
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Target: 5'- uCAAAAGGCACGCAAGGGACGACACAAu -3' miRNA: 3'- -GUUUUCCGUGCGUUCCCUGCUGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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