Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 324 | 5' | -52.4 | AC_000010.1 | + | 9262 | 0.67 | 0.622801 |
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Target: 5'- --aGGCGGGGGcGGAGGaGGAgGGg- -3' miRNA: 3'- auaCCGUCUCCaUCUCCaUCUgUCac -5' |
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| 324 | 5' | -52.4 | AC_000010.1 | + | 23511 | 0.68 | 0.587487 |
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Target: 5'- --gGGCAGAGGcGGAGGUGacugcGAUGGg- -3' miRNA: 3'- auaCCGUCUCCaUCUCCAU-----CUGUCac -5' |
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| 324 | 5' | -52.4 | AC_000010.1 | + | 8964 | 0.69 | 0.495898 |
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Target: 5'- --aGGCGcuGGaAGAGGUAGugAGUGu -3' miRNA: 3'- auaCCGUcuCCaUCUCCAUCugUCAC- -5' |
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| 324 | 5' | -52.4 | AC_000010.1 | + | 23831 | 0.72 | 0.36321 |
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Target: 5'- --gGGUAGAGGUGGuGGUggcgGGACugGGUGg -3' miRNA: 3'- auaCCGUCUCCAUCuCCA----UCUG--UCAC- -5' |
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| 324 | 5' | -52.4 | AC_000010.1 | + | 25968 | 0.76 | 0.199694 |
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Target: 5'- --aGGCAGAGGaGGAGGaGGACAGc- -3' miRNA: 3'- auaCCGUCUCCaUCUCCaUCUGUCac -5' |
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| 324 | 5' | -52.4 | AC_000010.1 | + | 25076 | 1.07 | 0.001311 |
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Target: 5'- gUAUGGCAGAGGUAGAGGUAGACAGUGu -3' miRNA: 3'- -AUACCGUCUCCAUCUCCAUCUGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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