Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32402 | 5' | -46 | NC_007056.1 | + | 7394 | 0.66 | 0.993088 |
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Target: 5'- aGCACgaugaUGCGAGAaaCAGUAGGUAUUGUu -3' miRNA: 3'- -CGUGg----AUGUUUUg-GUCAUUCAUGGCG- -5' |
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| 32402 | 5' | -46 | NC_007056.1 | + | 9547 | 0.68 | 0.978511 |
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Target: 5'- aUACCUAuCAuAGCUGGUuuAGUACCuGCg -3' miRNA: 3'- cGUGGAU-GUuUUGGUCAu-UCAUGG-CG- -5' |
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| 32402 | 5' | -46 | NC_007056.1 | + | 21462 | 0.68 | 0.972666 |
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Target: 5'- aCGCCUACAuguuuaauAACCuuaaUAGGUACUGUg -3' miRNA: 3'- cGUGGAUGUu-------UUGGuc--AUUCAUGGCG- -5' |
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| 32402 | 5' | -46 | NC_007056.1 | + | 10535 | 0.73 | 0.798772 |
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Target: 5'- uGUACUUACAAuAUUAGgcGGUGCUGUa -3' miRNA: 3'- -CGUGGAUGUUuUGGUCauUCAUGGCG- -5' |
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| 32402 | 5' | -46 | NC_007056.1 | + | 24095 | 0.74 | 0.787171 |
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Target: 5'- aGCACCUAUAAuACCAcugauuuGUucGAGUugUGCa -3' miRNA: 3'- -CGUGGAUGUUuUGGU-------CA--UUCAugGCG- -5' |
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| 32402 | 5' | -46 | NC_007056.1 | + | 24008 | 1.16 | 0.002646 |
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Target: 5'- aGCACCUACAAAACCAGUAAGUACCGCu -3' miRNA: 3'- -CGUGGAUGUUUUGGUCAUUCAUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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