Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32407 | 5' | -44 | NC_007065.1 | + | 10812 | 0.68 | 0.989421 |
Target: 5'- aUUUUG-CCCCACAauucaguuaacCAGCCAACu--ACa -3' miRNA: 3'- -AAAAUaGGGGUGU-----------GUUGGUUGuuuUG- -5' |
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32407 | 5' | -44 | NC_007065.1 | + | 25367 | 0.69 | 0.975659 |
Target: 5'- gUUUAUCCCUAgCACucUCAuuACAAGACg -3' miRNA: 3'- aAAAUAGGGGU-GUGuuGGU--UGUUUUG- -5' |
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32407 | 5' | -44 | NC_007065.1 | + | 15148 | 0.73 | 0.885374 |
Target: 5'- aUUUAUCCCCAUAU-ACCAauGCAGugauGCa -3' miRNA: 3'- aAAAUAGGGGUGUGuUGGU--UGUUu---UG- -5' |
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32407 | 5' | -44 | NC_007065.1 | + | 26685 | 1.08 | 0.011557 |
Target: 5'- uUUUUAUCCCCACACAACCAACAAAACc -3' miRNA: 3'- -AAAAUAGGGGUGUGUUGGUUGUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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