Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 325 | 5' | -58.4 | AC_000010.1 | + | 23827 | 0.66 | 0.403536 |
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Target: 5'- aGAGGUggUGGUGGCGGgAcuGGGUGGu- -3' miRNA: 3'- -CUCCG--GCCACUGCUgUuuCCCGCCuu -5' |
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| 325 | 5' | -58.4 | AC_000010.1 | + | 5880 | 0.66 | 0.37588 |
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Target: 5'- --aGCCGGUGGgGuauaaaAGGGGGCGGGu -3' miRNA: 3'- cucCGGCCACUgCug----UUUCCCGCCUu -5' |
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| 325 | 5' | -58.4 | AC_000010.1 | + | 8427 | 0.67 | 0.341047 |
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Target: 5'- aGGGCaCGGUGGUGGCGcccaAAGGGCacGGAAc -3' miRNA: 3'- cUCCG-GCCACUGCUGU----UUCCCG--CCUU- -5' |
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| 325 | 5' | -58.4 | AC_000010.1 | + | 85 | 0.68 | 0.285863 |
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Target: 5'- -uGGCUGuGggGACGGCGAccguuaGGGGCGGGg -3' miRNA: 3'- cuCCGGC-Ca-CUGCUGUU------UCCCGCCUu -5' |
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| 325 | 5' | -58.4 | AC_000010.1 | + | 35440 | 0.69 | 0.250924 |
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Target: 5'- -uGGCUGuGggGACGGCGAccguuaggggcGGGGCGGGAa -3' miRNA: 3'- cuCCGGC-Ca-CUGCUGUU-----------UCCCGCCUU- -5' |
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| 325 | 5' | -58.4 | AC_000010.1 | + | 27182 | 1.05 | 0.000468 |
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Target: 5'- uGAGGCCGGUGACGACAAAGGGCGGAAc -3' miRNA: 3'- -CUCCGGCCACUGCUGUUUCCCGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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