Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3254 | 5' | -46.7 | NC_001524.1 | + | 312 | 0.66 | 0.633848 |
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Target: 5'- ----cCCUGGAGACcuGUAUGAGCCc-- -3' miRNA: 3'- ccuaaGGAUUUCUG--UAUGCUCGGacg -5' |
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| 3254 | 5' | -46.7 | NC_001524.1 | + | 4878 | 0.66 | 0.633848 |
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Target: 5'- gGGcAUUCCggccuGGCAUaguggaagauGCaGGGCCUGCu -3' miRNA: 3'- -CC-UAAGGauuu-CUGUA----------UG-CUCGGACG- -5' |
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| 3254 | 5' | -46.7 | NC_001524.1 | + | 4673 | 0.67 | 0.606886 |
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Target: 5'- ---aUCCgGAGGACAggUGCcuGCCUGCu -3' miRNA: 3'- ccuaAGGaUUUCUGU--AUGcuCGGACG- -5' |
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| 3254 | 5' | -46.7 | NC_001524.1 | + | 7281 | 0.71 | 0.361346 |
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Target: 5'- ---aUCCUcaagaaucGAGGACGUAgGAGgCUGCa -3' miRNA: 3'- ccuaAGGA--------UUUCUGUAUgCUCgGACG- -5' |
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| 3254 | 5' | -46.7 | NC_001524.1 | + | 1216 | 0.72 | 0.32982 |
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Target: 5'- ---gUCUUGggaGGGACAUcuggaauccauuaACGAGCCUGCu -3' miRNA: 3'- ccuaAGGAU---UUCUGUA-------------UGCUCGGACG- -5' |
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| 3254 | 5' | -46.7 | NC_001524.1 | + | 5413 | 1.04 | 0.00158 |
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Target: 5'- aGGAUUCUcAAAGACAUACGAGCCUGCa -3' miRNA: 3'- -CCUAAGGaUUUCUGUAUGCUCGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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