Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3256 | 3' | -58.7 | NC_001531.1 | + | 3677 | 0.7 | 0.053647 |
Target: 5'- uGCAGaAGG-GGAGGUGGAaGGUcACCc -3' miRNA: 3'- -CGUCcUCCuCCUCCACCUcCCAcUGG- -5' |
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3256 | 3' | -58.7 | NC_001531.1 | + | 3886 | 0.83 | 0.004377 |
Target: 5'- gGCGGG-GGAGGAGGaGGAGGaUGACCu -3' miRNA: 3'- -CGUCCuCCUCCUCCaCCUCCcACUGG- -5' |
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3256 | 3' | -58.7 | NC_001531.1 | + | 3750 | 1.13 | 1.2e-05 |
Target: 5'- uGCAGGAGGAGGAGGUGGAGGGUGACCu -3' miRNA: 3'- -CGUCCUCCUCCUCCACCUCCCACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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