Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32563 | 5' | -40 | NC_007149.1 | + | 29697 | 0.66 | 0.999996 |
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Target: 5'- gGAAGACUUACGGAAGauuCAAGAa----- -3' miRNA: 3'- -CUUUUGAAUGUCUUU---GUUCUcagaag -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 21035 | 0.68 | 0.999873 |
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Target: 5'- -----aUUGCAGGAAUAAGAGcagcaucaagaccauUCUUCa -3' miRNA: 3'- cuuuugAAUGUCUUUGUUCUC---------------AGAAG- -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 33851 | 0.69 | 0.999739 |
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Target: 5'- aGAAACUuuagcUGCAuuAGC-AGAGUCUUCa -3' miRNA: 3'- cUUUUGA-----AUGUcuUUGuUCUCAGAAG- -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 38808 | 0.69 | 0.999546 |
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Target: 5'- gGGAAGcCUUAU-GGGACAAGGGUuCUUCu -3' miRNA: 3'- -CUUUU-GAAUGuCUUUGUUCUCA-GAAG- -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 26045 | 0.7 | 0.999027 |
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Target: 5'- aGAAGACgau--GAAACucAGGGUCUUCa -3' miRNA: 3'- -CUUUUGaauguCUUUGu-UCUCAGAAG- -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 13285 | 0.7 | 0.998768 |
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Target: 5'- uGAAGCUUAuCAGAAAgAAG-GUCUUg -3' miRNA: 3'- cUUUUGAAU-GUCUUUgUUCuCAGAAg -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 28743 | 0.71 | 0.997609 |
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Target: 5'- aGAGAGCUgu-AGAGGcCAAGAaGUCUUCu -3' miRNA: 3'- -CUUUUGAaugUCUUU-GUUCU-CAGAAG- -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 19774 | 0.72 | 0.995656 |
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Target: 5'- -uAGACgUACAGGAauccaaggGCAAGAGUCUa- -3' miRNA: 3'- cuUUUGaAUGUCUU--------UGUUCUCAGAag -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 37790 | 0.78 | 0.889755 |
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Target: 5'- uGAAGACUUACGGGAACGuGAacuGUCUUa -3' miRNA: 3'- -CUUUUGAAUGUCUUUGUuCU---CAGAAg -5' |
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| 32563 | 5' | -40 | NC_007149.1 | + | 38842 | 1.13 | 0.018762 |
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Target: 5'- gGAAAACUUACAGAAACAAGAGUCUUCu -3' miRNA: 3'- -CUUUUGAAUGUCUUUGUUCUCAGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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