Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32568 | 5' | -43.8 | NC_007151.1 | + | 31307 | 0.66 | 1 |
Target: 5'- ---uUCGAUGACGAUauauuuuUGAGAGc -3' miRNA: 3'- uaacAGCUACUGCUAaaacu--ACUCUC- -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 93983 | 0.66 | 1 |
Target: 5'- --cGaCGA-GACGAcauucUUUGAUGAGAGa -3' miRNA: 3'- uaaCaGCUaCUGCUa----AAACUACUCUC- -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 58232 | 0.66 | 1 |
Target: 5'- -aUGUCGGUGACugcucccucucacUUUGAUGaAGAGg -3' miRNA: 3'- uaACAGCUACUGcua----------AAACUAC-UCUC- -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 18112 | 0.66 | 0.999999 |
Target: 5'- aAUUGUCGAUGACGAUc-UGAa----- -3' miRNA: 3'- -UAACAGCUACUGCUAaaACUacucuc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 94716 | 0.68 | 0.999979 |
Target: 5'- ----cCGAUGACGAUUaUGAUGAa-- -3' miRNA: 3'- uaacaGCUACUGCUAAaACUACUcuc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 121992 | 0.68 | 0.999971 |
Target: 5'- --aGUCGAacucaUGcCGGUUUUGAcGAGAGc -3' miRNA: 3'- uaaCAGCU-----ACuGCUAAAACUaCUCUC- -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 26225 | 0.69 | 0.999956 |
Target: 5'- -aUGUCGAUGACGuuucguucgaaacaAUg--GAUGAGGa -3' miRNA: 3'- uaACAGCUACUGC--------------UAaaaCUACUCUc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 119067 | 0.69 | 0.999927 |
Target: 5'- -aUGgUGAUGAUGGUggUGGUGAGGa -3' miRNA: 3'- uaACaGCUACUGCUAaaACUACUCUc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 126128 | 0.71 | 0.999541 |
Target: 5'- uUUGUCGAUGACGGUggca--GAGAu -3' miRNA: 3'- uAACAGCUACUGCUAaaacuaCUCUc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 39711 | 0.71 | 0.999275 |
Target: 5'- -aUGUUGAUGAUGAUgaugUUGAuuuuguUGAGAa -3' miRNA: 3'- uaACAGCUACUGCUAa---AACU------ACUCUc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 27393 | 0.78 | 0.939362 |
Target: 5'- -aUGUCGAUGAUGAUUUcGAUGAcGAc -3' miRNA: 3'- uaACAGCUACUGCUAAAaCUACU-CUc -5' |
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32568 | 5' | -43.8 | NC_007151.1 | + | 27534 | 1.08 | 0.051337 |
Target: 5'- aAUUGUCGAUGACGAUUUUGAUGAGAGu -3' miRNA: 3'- -UAACAGCUACUGCUAAAACUACUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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