Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32569 | 3' | -48.9 | NC_007151.1 | + | 31660 | 0.66 | 0.999145 |
Target: 5'- aUUGAuguucggucaGGUCGuuUCCUCuaGUGGCAUCGa -3' miRNA: 3'- cAACU----------UUAGCucAGGAG--CGCUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 67429 | 0.66 | 0.999127 |
Target: 5'- --cGAAGaCGAGUugcgucagaaauaUCUCGCcGACGUCGa -3' miRNA: 3'- caaCUUUaGCUCA-------------GGAGCG-CUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 145758 | 0.67 | 0.998143 |
Target: 5'- uUUGucuUUGGcGUCCUCGaCGGCAUCu -3' miRNA: 3'- cAACuuuAGCU-CAGGAGC-GCUGUAGc -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 45710 | 0.67 | 0.998143 |
Target: 5'- uUUGccacucgCGAGcCgUCGCGGCAUCGu -3' miRNA: 3'- cAACuuua---GCUCaGgAGCGCUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 123098 | 0.68 | 0.995655 |
Target: 5'- aUUGAAGUCGAcGUCgUCGacguaaaucuCGAUGUCGa -3' miRNA: 3'- cAACUUUAGCU-CAGgAGC----------GCUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 21095 | 0.68 | 0.992108 |
Target: 5'- uUUGAGcGUCGAGuUUCUCGUucaauuccguGACAUCGg -3' miRNA: 3'- cAACUU-UAGCUC-AGGAGCG----------CUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 106945 | 0.7 | 0.978459 |
Target: 5'- -aUGu--UCGAGUCUU-GCGACAUCa -3' miRNA: 3'- caACuuuAGCUCAGGAgCGCUGUAGc -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 113946 | 0.7 | 0.978459 |
Target: 5'- -aUGAAAUUGAG-CUUCGCGAUAa-- -3' miRNA: 3'- caACUUUAGCUCaGGAGCGCUGUagc -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 1261 | 0.71 | 0.960199 |
Target: 5'- -gUGGAAUCGaAGUCa--GUGGCAUCGg -3' miRNA: 3'- caACUUUAGC-UCAGgagCGCUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 76173 | 0.72 | 0.943311 |
Target: 5'- aGUUGGGAUCGAGcgagaUCaCUaGCGACAUCu -3' miRNA: 3'- -CAACUUUAGCUC-----AG-GAgCGCUGUAGc -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 35140 | 0.75 | 0.865713 |
Target: 5'- --cGGAAUCGAGcUCCUCGUcguagcagcucacgGGCAUCGc -3' miRNA: 3'- caaCUUUAGCUC-AGGAGCG--------------CUGUAGC- -5' |
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32569 | 3' | -48.9 | NC_007151.1 | + | 31882 | 1 | 0.055245 |
Target: 5'- uGUUGAAAUCGAGUCCUCGCGACA-CGa -3' miRNA: 3'- -CAACUUUAGCUCAGGAGCGCUGUaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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