Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32572 | 3' | -46.6 | NC_007151.1 | + | 80565 | 0.66 | 0.999875 |
Target: 5'- -uCGACAGCACcGGUcUCGUuGCcaacgUGCc -3' miRNA: 3'- uuGCUGUCGUGuUUAuAGCA-CGa----ACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 114621 | 0.66 | 0.999836 |
Target: 5'- cGACGACAGC-C-AAUGUCGUGa---- -3' miRNA: 3'- -UUGCUGUCGuGuUUAUAGCACgaacg -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 135131 | 0.66 | 0.999836 |
Target: 5'- uAACGACAGCACuauuauAGUuuucAUUGUGCa--- -3' miRNA: 3'- -UUGCUGUCGUGu-----UUA----UAGCACGaacg -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 52207 | 0.66 | 0.999832 |
Target: 5'- -uCGACAuguaucuGCGCAAucAUAUCGUGgCaUGCg -3' miRNA: 3'- uuGCUGU-------CGUGUU--UAUAGCAC-GaACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 106238 | 0.66 | 0.999727 |
Target: 5'- uGACGAUGGCAaGAAUAUUGUcggguaGCaUGCg -3' miRNA: 3'- -UUGCUGUCGUgUUUAUAGCA------CGaACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 110359 | 0.66 | 0.999727 |
Target: 5'- -cCGACAcGUACAAauGUGUCGUGaugaaaUGCg -3' miRNA: 3'- uuGCUGU-CGUGUU--UAUAGCACga----ACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 26498 | 0.67 | 0.999559 |
Target: 5'- uAGCGACGGCAUcgGUggCGgugGCggugGCg -3' miRNA: 3'- -UUGCUGUCGUGuuUAuaGCa--CGaa--CG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 30897 | 0.68 | 0.998077 |
Target: 5'- cGACGACAGCAUGAuugauuUCGgauuaauuUGUUUGCc -3' miRNA: 3'- -UUGCUGUCGUGUUuau---AGC--------ACGAACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 22954 | 0.69 | 0.997226 |
Target: 5'- uGCGGguGCAUu--UGUCGUGUUUGa -3' miRNA: 3'- uUGCUguCGUGuuuAUAGCACGAACg -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 67272 | 0.69 | 0.996083 |
Target: 5'- gAACGAguCAGC-CAA--AUCGUGCgUGCg -3' miRNA: 3'- -UUGCU--GUCGuGUUuaUAGCACGaACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 55067 | 0.69 | 0.995381 |
Target: 5'- uGCGGCGGCGgcGGUGUCGacgucGUUUGCg -3' miRNA: 3'- uUGCUGUCGUguUUAUAGCa----CGAACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 43600 | 0.7 | 0.994581 |
Target: 5'- -uCGGCGGUGgGuAUAUCGUGgUUGCu -3' miRNA: 3'- uuGCUGUCGUgUuUAUAGCACgAACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 71032 | 0.7 | 0.993671 |
Target: 5'- cACGACcGCACAAAUGUCaUGUUcacGCa -3' miRNA: 3'- uUGCUGuCGUGUUUAUAGcACGAa--CG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 102252 | 0.71 | 0.987135 |
Target: 5'- -uCGACGGCACcGAaAUCGcUGCUcGCg -3' miRNA: 3'- uuGCUGUCGUGuUUaUAGC-ACGAaCG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 70821 | 0.71 | 0.983397 |
Target: 5'- cGACGGCAGCGCGGAUcuaGUGUUgaacgacugggUGCu -3' miRNA: 3'- -UUGCUGUCGUGUUUAuagCACGA-----------ACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 43664 | 0.71 | 0.983397 |
Target: 5'- cGACGACGGCGCGuuaGUCGU--UUGCu -3' miRNA: 3'- -UUGCUGUCGUGUuuaUAGCAcgAACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 29490 | 0.72 | 0.973535 |
Target: 5'- uACGACAGCACcgucGAAUAUagcgacaGUGCcgUGCu -3' miRNA: 3'- uUGCUGUCGUG----UUUAUAg------CACGa-ACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 146954 | 0.74 | 0.951693 |
Target: 5'- cGACGAUgaaGGCACGgugAAUugaAUCGUGUUUGCu -3' miRNA: 3'- -UUGCUG---UCGUGU---UUA---UAGCACGAACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 65617 | 0.81 | 0.640115 |
Target: 5'- cGACGACAGUGCcGAUGUUGUGCUgaucgauugggUGCg -3' miRNA: 3'- -UUGCUGUCGUGuUUAUAGCACGA-----------ACG- -5' |
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32572 | 3' | -46.6 | NC_007151.1 | + | 65829 | 1.12 | 0.013536 |
Target: 5'- aAACGACAGCACAAAUAUCGUGCUUGCg -3' miRNA: 3'- -UUGCUGUCGUGUUUAUAGCACGAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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