Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32572 | 5' | -51.4 | NC_007151.1 | + | 65168 | 0.66 | 0.991724 |
Target: 5'- -cGUCAaagACGACAUCGGCACaGUUGu -3' miRNA: 3'- gcUAGUcg-UGUUGUAGCCGUGaCAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 55081 | 0.66 | 0.991724 |
Target: 5'- uCGAUauaaaAGUuuGCGGCggCGGCGgUGUCGa -3' miRNA: 3'- -GCUAg----UCG--UGUUGuaGCCGUgACAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 114443 | 0.66 | 0.989189 |
Target: 5'- -uGUCAuaauuCACGACAUUGGCugUcGUCGg -3' miRNA: 3'- gcUAGUc----GUGUUGUAGCCGugA-CAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 127851 | 0.66 | 0.989189 |
Target: 5'- cCGAUCuuuGUAUAcGCAgacaCGGCACUGUUc -3' miRNA: 3'- -GCUAGu--CGUGU-UGUa---GCCGUGACAGc -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 80381 | 0.67 | 0.984299 |
Target: 5'- aGAagGGCACGuugGCAacgagacCGGUGCUGUCGa -3' miRNA: 3'- gCUagUCGUGU---UGUa------GCCGUGACAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 118030 | 0.67 | 0.982343 |
Target: 5'- ----aAGCACGGCAUCGGCAUccaagCGg -3' miRNA: 3'- gcuagUCGUGUUGUAGCCGUGaca--GC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 20667 | 0.68 | 0.969728 |
Target: 5'- ---aCGGCAcCGACGaCGGUAUUGUCGu -3' miRNA: 3'- gcuaGUCGU-GUUGUaGCCGUGACAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 65643 | 0.68 | 0.966583 |
Target: 5'- uCGAUUgggugcgcaAGCACGAUAUUuGUGCUGUCGu -3' miRNA: 3'- -GCUAG---------UCGUGUUGUAGcCGUGACAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 134306 | 0.69 | 0.947387 |
Target: 5'- -uGUCGGCGCcGCcguaGUCGGCGaUGUCGg -3' miRNA: 3'- gcUAGUCGUGuUG----UAGCCGUgACAGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 89442 | 0.7 | 0.921464 |
Target: 5'- -aAUCGGCACAaucccucuccagcACAUCGGUAUUGaCGa -3' miRNA: 3'- gcUAGUCGUGU-------------UGUAGCCGUGACaGC- -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 107579 | 0.84 | 0.283391 |
Target: 5'- uGGUCGGCaacgucgacgACAACGUCGGCGCUGUUa -3' miRNA: 3'- gCUAGUCG----------UGUUGUAGCCGUGACAGc -5' |
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32572 | 5' | -51.4 | NC_007151.1 | + | 65794 | 0.99 | 0.034008 |
Target: 5'- uCGA-CAGCACAACAUCGGCACUGUCGu -3' miRNA: 3'- -GCUaGUCGUGUUGUAGCCGUGACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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