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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 32573 | 3' | -39.8 | NC_007151.1 | + | 41415 | 0.66 | 1 |
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Target: 5'- aUGAGACGUCAAAAuGAaaUGUCUc--- -3' miRNA: 3'- cGCUUUGCAGUUUUuCU--AUAGGuaac -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 71920 | 0.66 | 1 |
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Target: 5'- cGCGAc-CGUCGAGAcuGUAUCUAUc- -3' miRNA: 3'- -CGCUuuGCAGUUUUucUAUAGGUAac -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 78154 | 0.67 | 1 |
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Target: 5'- gGCGGAucggccguCGUCGAAGAG-UGUCCc--- -3' miRNA: 3'- -CGCUUu-------GCAGUUUUUCuAUAGGuaac -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 26798 | 0.67 | 1 |
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Target: 5'- aCGAGACGUUucguGAGGAUuugcGUCgAUUGa -3' miRNA: 3'- cGCUUUGCAGuu--UUUCUA----UAGgUAAC- -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 128853 | 0.68 | 1 |
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Target: 5'- uCGAAGCGuUCGAGAGGAUacaggacaggcucGUCCGc-- -3' miRNA: 3'- cGCUUUGC-AGUUUUUCUA-------------UAGGUaac -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 60274 | 0.7 | 1 |
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Target: 5'- uGCGAAACGUaCAAAucGAU-UCUAUUc -3' miRNA: 3'- -CGCUUUGCA-GUUUuuCUAuAGGUAAc -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 99204 | 0.71 | 1 |
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Target: 5'- -aGAAACuGUUAAucAGAUAUCCGUa- -3' miRNA: 3'- cgCUUUG-CAGUUuuUCUAUAGGUAac -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 26911 | 0.71 | 1 |
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Target: 5'- aGCGAcuauAACauUCAAAAAGAaucuacUAUCCAUUGg -3' miRNA: 3'- -CGCU----UUGc-AGUUUUUCU------AUAGGUAAC- -5' |
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| 32573 | 3' | -39.8 | NC_007151.1 | + | 78358 | 1.15 | 0.063298 |
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Target: 5'- uGCGAAACGUCAAAAAGAUAUCCAUUGg -3' miRNA: 3'- -CGCUUUGCAGUUUUUCUAUAGGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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