Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32575 | 3' | -43.9 | NC_007151.1 | + | 68064 | 0.66 | 1 |
Target: 5'- aCGCUUUGAaucgcuUCGCGGCAggCAUUGUg- -3' miRNA: 3'- aGUGAAAUU------GGCGUUGUa-GUAGCAau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 49751 | 0.66 | 1 |
Target: 5'- aCGCUUUGAaucgcuUCGCGGCAggCAUUGUg- -3' miRNA: 3'- aGUGAAAUU------GGCGUUGUa-GUAGCAau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 77297 | 0.66 | 1 |
Target: 5'- aUCACU---GCCGuCGACGUCAaCGg-- -3' miRNA: 3'- -AGUGAaauUGGC-GUUGUAGUaGCaau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 45717 | 0.67 | 0.999994 |
Target: 5'- cUCGCg--AGCCGUcGCggCAUCGUUc -3' miRNA: 3'- -AGUGaaaUUGGCGuUGuaGUAGCAAu -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 59460 | 0.67 | 0.999994 |
Target: 5'- -aACUauaUAACCGCAACAcgAUCGUUu -3' miRNA: 3'- agUGAa--AUUGGCGUUGUagUAGCAAu -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 1787 | 0.68 | 0.999987 |
Target: 5'- -gACgUUGGCCGCAACAUCGauaGUg- -3' miRNA: 3'- agUGaAAUUGGCGUUGUAGUag-CAau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 95217 | 0.68 | 0.999975 |
Target: 5'- aUAgUUUGAUCGUgucGAUGUCAUCGUUGu -3' miRNA: 3'- aGUgAAAUUGGCG---UUGUAGUAGCAAU- -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 101199 | 0.69 | 0.999915 |
Target: 5'- -------cACCGCAACAUUGUCGUa- -3' miRNA: 3'- agugaaauUGGCGUUGUAGUAGCAau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 48709 | 0.7 | 0.999748 |
Target: 5'- cCGCUUUAG-CGCGACAU--UCGUUAg -3' miRNA: 3'- aGUGAAAUUgGCGUUGUAguAGCAAU- -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 93532 | 0.71 | 0.999345 |
Target: 5'- aUCAgacUAGCCGaCGACAUUGUCGUUGa -3' miRNA: 3'- -AGUgaaAUUGGC-GUUGUAGUAGCAAU- -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 35268 | 0.71 | 0.998987 |
Target: 5'- -aGCUUUGACaugaggcuaGUGACGUCAUCGUUu -3' miRNA: 3'- agUGAAAUUGg--------CGUUGUAGUAGCAAu -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 26730 | 0.71 | 0.998987 |
Target: 5'- cCACcagcuACCGCAACAUUggCGUUAa -3' miRNA: 3'- aGUGaaau-UGGCGUUGUAGuaGCAAU- -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 12949 | 0.74 | 0.992705 |
Target: 5'- -uGCUaUAGCCGCAGCAU--UCGUUGg -3' miRNA: 3'- agUGAaAUUGGCGUUGUAguAGCAAU- -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 120396 | 0.78 | 0.935937 |
Target: 5'- uUCGCU---GCCGcCGACGUCAUCGUg- -3' miRNA: 3'- -AGUGAaauUGGC-GUUGUAGUAGCAau -5' |
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32575 | 3' | -43.9 | NC_007151.1 | + | 105988 | 1.09 | 0.040337 |
Target: 5'- aUCACUUUAACCGCAACAUCAUCGUUAu -3' miRNA: 3'- -AGUGAAAUUGGCGUUGUAGUAGCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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