Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32578 | 5' | -49.5 | NC_007151.1 | + | 16692 | 0.67 | 0.995337 |
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Target: 5'- cAGCAAUaUUCU--GGAUUUCGGCaACGa -3' miRNA: 3'- -UCGUUA-GAGAuaUCUGAAGCCGgUGC- -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 142244 | 0.67 | 0.992678 |
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Target: 5'- cGGCAAUCUUU---GACagCGGCgGCGa -3' miRNA: 3'- -UCGUUAGAGAuauCUGaaGCCGgUGC- -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 31649 | 0.67 | 0.991562 |
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Target: 5'- cAGCAAUCUCgauuGAUgUUCGGUCAgGu -3' miRNA: 3'- -UCGUUAGAGauauCUG-AAGCCGGUgC- -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 51376 | 0.69 | 0.974504 |
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Target: 5'- --uGGUCUUUGUcGAUUUCGGCgGCGg -3' miRNA: 3'- ucgUUAGAGAUAuCUGAAGCCGgUGC- -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 43879 | 0.71 | 0.940365 |
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Target: 5'- cAGacauGUCUCUGgAGACUUUGGCCGa- -3' miRNA: 3'- -UCgu--UAGAGAUaUCUGAAGCCGGUgc -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 62059 | 0.71 | 0.929996 |
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Target: 5'- cGCAuguUCUCUAUGGAUUUauuccuauaugCGGCgGCGa -3' miRNA: 3'- uCGUu--AGAGAUAUCUGAA-----------GCCGgUGC- -5' |
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| 32578 | 5' | -49.5 | NC_007151.1 | + | 118880 | 1.1 | 0.00932 |
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Target: 5'- aAGCAAUCUCUAUAGACUUCGGCCACGa -3' miRNA: 3'- -UCGUUAGAGAUAUCUGAAGCCGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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