miRNA: 3'- gAG-CCGAGG---------UUACCACa---GCCUa----UGCU- -5'
miRNA: 3'- gaGCCGAGGuuacCACA----GCCUAUGCU- -5'
miRNA: 3'- gAGCCGAGGUUAcCA--CAGCCuaUGCU- -5'
miRNA: 3'- -GAGCCGAGGUUACCACAGCCUAUGCU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32582 | 3' | -53 | NC_007151.1 | + | 79699 | 0.66 | 0.978061 |
|
Target: 5'- uUCaGGCUCUgguaugacuGGUGGUGguggCGGAggaggACGAg -3' miRNA: 3'- gAG-CCGAGG---------UUACCACa---GCCUa----UGCU- -5' |
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| 32582 | 3' | -53 | NC_007151.1 | + | 81161 | 0.66 | 0.975598 |
|
Target: 5'- -aCGGCUCUcc--GUGUucucCGGAUGCGAa -3' miRNA: 3'- gaGCCGAGGuuacCACA----GCCUAUGCU- -5' |
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| 32582 | 3' | -53 | NC_007151.1 | + | 30623 | 0.68 | 0.939132 |
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Target: 5'- aUUGGCUUCAAUuGUacGUCGGcgACGAu -3' miRNA: 3'- gAGCCGAGGUUAcCA--CAGCCuaUGCU- -5' |
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| 32582 | 3' | -53 | NC_007151.1 | + | 130349 | 1.09 | 0.005893 |
|
Target: 5'- gCUCGGCUCCAAUGGUGUCGGAUACGAc -3' miRNA: 3'- -GAGCCGAGGUUACCACAGCCUAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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