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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32582 | 5' | -48.4 | NC_007151.1 | + | 138508 | 0.66 | 0.999411 |
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Target: 5'- uGCCGUUCGCcucgauUCGUUCgucuccaucaGCACCa -3' miRNA: 3'- gUGGUAAGCGcuuu--AGUAAG----------CGUGGc -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 29374 | 0.67 | 0.998661 |
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Target: 5'- uGCUAUUCGCGAGuUUAUcgaggagacUgGCACCu -3' miRNA: 3'- gUGGUAAGCGCUUuAGUA---------AgCGUGGc -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 130314 | 1.11 | 0.012579 |
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Target: 5'- aCACCAUUCGCGAAAUCAUUCGCACCGa -3' miRNA: 3'- -GUGGUAAGCGCUUUAGUAAGCGUGGC- -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 14920 | 0.73 | 0.930879 |
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Target: 5'- aCGCCAcUCGCGAGAUCGU---UACCGu -3' miRNA: 3'- -GUGGUaAGCGCUUUAGUAagcGUGGC- -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 43929 | 0.7 | 0.979917 |
|
Target: 5'- uCACCA-UCGaCGGA--CGUUUGCACCGu -3' miRNA: 3'- -GUGGUaAGC-GCUUuaGUAAGCGUGGC- -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 80243 | 0.68 | 0.996706 |
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Target: 5'- gCACCAUcaUCG-GAuAUUGUUCGUAUCGg -3' miRNA: 3'- -GUGGUA--AGCgCUuUAGUAAGCGUGGC- -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 84794 | 0.67 | 0.998661 |
|
Target: 5'- aACCGUUgGCGuu-UCGUUUGCugACCa -3' miRNA: 3'- gUGGUAAgCGCuuuAGUAAGCG--UGGc -5' |
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| 32582 | 5' | -48.4 | NC_007151.1 | + | 86396 | 0.66 | 0.99927 |
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Target: 5'- aCACCAaUCGUGAuca-AUUCGCuaugauCCGa -3' miRNA: 3'- -GUGGUaAGCGCUuuagUAAGCGu-----GGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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