Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32583 | 3' | -50.7 | NC_007151.1 | + | 95430 | 0.72 | 0.858807 |
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Target: 5'- --cGCGAccaacgUCgACGUCAGGGCGCUUu -3' miRNA: 3'- aguCGCUaua---AG-UGCAGUUCCGCGAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 54098 | 0.69 | 0.963256 |
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Target: 5'- aCAGCGcuuGUAUuuaaUCACGUUAAuGCGUUCg -3' miRNA: 3'- aGUCGC---UAUA----AGUGCAGUUcCGCGAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 19290 | 0.67 | 0.982568 |
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Target: 5'- gCGGCGGcua-CGCGaCAAGGCGCa- -3' miRNA: 3'- aGUCGCUauaaGUGCaGUUCCGCGag -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 62146 | 0.67 | 0.987948 |
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Target: 5'- uUCAGCGGUcguGUUCGgGUCGAGaCGaCUUu -3' miRNA: 3'- -AGUCGCUA---UAAGUgCAGUUCcGC-GAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 114103 | 0.67 | 0.987948 |
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Target: 5'- cCAGUGAUGUacUCuuccuUGUCGGGuCGCUCg -3' miRNA: 3'- aGUCGCUAUA--AGu----GCAGUUCcGCGAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 94907 | 0.68 | 0.975552 |
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Target: 5'- aUCGGCGGUGUUgAUGUCGAcGCcUUCa -3' miRNA: 3'- -AGUCGCUAUAAgUGCAGUUcCGcGAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 69829 | 0.7 | 0.942068 |
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Target: 5'- -aAGCGAUcgAUUUACGUCAAuGGCucgcuucgaccguGCUCa -3' miRNA: 3'- agUCGCUA--UAAGUGCAGUU-CCG-------------CGAG- -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 95898 | 0.76 | 0.665278 |
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Target: 5'- gUAGCGAUcggcUUCgAUGUCGAGGCGCUa -3' miRNA: 3'- aGUCGCUAu---AAG-UGCAGUUCCGCGAg -5' |
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| 32583 | 3' | -50.7 | NC_007151.1 | + | 133784 | 1.12 | 0.005656 |
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Target: 5'- gUCAGCGAUAUUCACGUCAAGGCGCUCg -3' miRNA: 3'- -AGUCGCUAUAAGUGCAGUUCCGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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