miRNA: 3'- cucuuaCCC----AGUAGCUGCACCUG---UAG- -5'
miRNA: 3'- cUCUUACCCaGUAGCUGCa-----C-CUGUAG- -5'
miRNA: 3'- -CUCUUACCCAGUAGCUGCACCUGUAG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32583 | 5' | -51 | NC_007151.1 | + | 140543 | 0.68 | 0.965749 |
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Target: 5'- -----cGGGacgaUCGUCGGCGUGGAUggaGUCg -3' miRNA: 3'- cucuuaCCC----AGUAGCUGCACCUG---UAG- -5' |
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| 32583 | 5' | -51 | NC_007151.1 | + | 127062 | 0.72 | 0.87337 |
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Target: 5'- uAGAGUGGG-UAUCGGCGgaggcgGcGGCAUCg -3' miRNA: 3'- cUCUUACCCaGUAGCUGCa-----C-CUGUAG- -5' |
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| 32583 | 5' | -51 | NC_007151.1 | + | 133748 | 1.11 | 0.00608 |
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Target: 5'- cGAGAAUGGGUCAUCGACGUGGACAUCg -3' miRNA: 3'- -CUCUUACCCAGUAGCUGCACCUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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