miRNA: 3'- -CGgaCGCGUUUAGCUCaCAGCUGucu -5'
miRNA: 3'- -CGGaCGCGUu-----UAGCU--CACAGCuGUcu -5'
miRNA: 3'- -CGgACGCGUUuaGCu--------CACAGCUGUCU- -5'
miRNA: 3'- cGGACGCGuuuagCUCACAGCUGUcu -5'
miRNA: 3'- -CGGACGCGUUUAGCUCACAGCUGUCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32584 | 5' | -51.4 | NC_007151.1 | + | 58738 | 0.66 | 0.991724 |
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Target: 5'- gGCg-GCGCGAGUCGAGaGUUGAUu-- -3' miRNA: 3'- -CGgaCGCGUUUAGCUCaCAGCUGucu -5' |
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| 32584 | 5' | -51.4 | NC_007151.1 | + | 60814 | 0.66 | 0.986086 |
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Target: 5'- cGCCgGCGCAucccauGUCGAugGUGUCG-CAa- -3' miRNA: 3'- -CGGaCGCGUu-----UAGCU--CACAGCuGUcu -5' |
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| 32584 | 5' | -51.4 | NC_007151.1 | + | 16253 | 0.69 | 0.94282 |
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Target: 5'- aGCgUGUGCGAggCGugaccguauGUGUCGAUGGAa -3' miRNA: 3'- -CGgACGCGUUuaGCu--------CACAGCUGUCU- -5' |
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| 32584 | 5' | -51.4 | NC_007151.1 | + | 113548 | 0.71 | 0.876009 |
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Target: 5'- cCCUGCGUua---GAGUGUCGACGc- -3' miRNA: 3'- cGGACGCGuuuagCUCACAGCUGUcu -5' |
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| 32584 | 5' | -51.4 | NC_007151.1 | + | 136786 | 1.12 | 0.005202 |
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Target: 5'- cGCCUGCGCAAAUCGAGUGUCGACAGAu -3' miRNA: 3'- -CGGACGCGUUUAGCUCACAGCUGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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