miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32587 3' -51.1 NC_007151.1 + 134069 0.67 0.977331
Target:  5'- gAUAUCGUCAuGCGcGGUGCUguUGAggCGUg -3'
miRNA:   3'- -UAUAGUAGU-CGC-CCGCGA--ACUa-GUAg -5'
32587 3' -51.1 NC_007151.1 + 12048 0.69 0.932288
Target:  5'- -aGUCAUCAuCGGGCGauugucaugaacacuUUUGAUUAUCa -3'
miRNA:   3'- uaUAGUAGUcGCCCGC---------------GAACUAGUAG- -5'
32587 3' -51.1 NC_007151.1 + 109462 0.7 0.906124
Target:  5'- aAUGUCGUCAGCGacuauGGCGUcgacgaguccucUUGAUCuGUCg -3'
miRNA:   3'- -UAUAGUAGUCGC-----CCGCG------------AACUAG-UAG- -5'
32587 3' -51.1 NC_007151.1 + 49531 0.7 0.898783
Target:  5'- uUAUCaAUCAaGCGGGCGUcuucgagUUGAUCAa- -3'
miRNA:   3'- uAUAG-UAGU-CGCCCGCG-------AACUAGUag -5'
32587 3' -51.1 NC_007151.1 + 4657 0.84 0.235553
Target:  5'- ------nCAGCGGGUGCUUGAUCAUCa -3'
miRNA:   3'- uauaguaGUCGCCCGCGAACUAGUAG- -5'
32587 3' -51.1 NC_007151.1 + 4632 1.09 0.00659
Target:  5'- aAUAUCAUCAGCGGGCGCUUGAUCAUCa -3'
miRNA:   3'- -UAUAGUAGUCGCCCGCGAACUAGUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.