Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32589 | 5' | -45.9 | NC_007151.1 | + | 85511 | 0.66 | 0.999849 |
Target: 5'- -cGAUUUUcCCGAGcCCGGCuuGCCGUu -3' miRNA: 3'- uuUUAAAAaGGCUUaGGUCGu-CGGUA- -5' |
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32589 | 5' | -45.9 | NC_007151.1 | + | 69493 | 0.67 | 0.999579 |
Target: 5'- ------gUUCUGAAUCCAGUAGUUu- -3' miRNA: 3'- uuuuaaaAAGGCUUAGGUCGUCGGua -5' |
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32589 | 5' | -45.9 | NC_007151.1 | + | 134495 | 0.68 | 0.999164 |
Target: 5'- -------aUCCGAAUCCGGCAuUCAa -3' miRNA: 3'- uuuuaaaaAGGCUUAGGUCGUcGGUa -5' |
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32589 | 5' | -45.9 | NC_007151.1 | + | 67398 | 1.03 | 0.043961 |
Target: 5'- aAAAAUUUUUCCGAAUCCAGCAGCCAUu -3' miRNA: 3'- -UUUUAAAAAGGCUUAGGUCGUCGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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