Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32591 | 5' | -50.2 | NC_007151.1 | + | 51768 | 0.66 | 0.992181 |
Target: 5'- aCAUCGGUCAguaaguAGGGGAUUCUgucuccguugccgGGUAGAAa -3' miRNA: 3'- -GUGGCCGGU------UCCUUUAAGA-------------CCGUUUU- -5' |
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32591 | 5' | -50.2 | NC_007151.1 | + | 66629 | 0.66 | 0.991116 |
Target: 5'- aCGCCGGCCAAucuAAUguguuugucUUUGGCGAAAu -3' miRNA: 3'- -GUGGCCGGUUccuUUA---------AGACCGUUUU- -5' |
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32591 | 5' | -50.2 | NC_007151.1 | + | 71519 | 0.73 | 0.812682 |
Target: 5'- -uUgGGCCAAGGAAAcUCUGGUGAu- -3' miRNA: 3'- guGgCCGGUUCCUUUaAGACCGUUuu -5' |
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32591 | 5' | -50.2 | NC_007151.1 | + | 124779 | 1.06 | 0.011487 |
Target: 5'- aCACCGGCCAAGGAAAUUCUGGCAAAAc -3' miRNA: 3'- -GUGGCCGGUUCCUUUAAGACCGUUUU- -5' |
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32591 | 5' | -50.2 | NC_007151.1 | + | 126796 | 0.68 | 0.975933 |
Target: 5'- -uCCGGCCAuAGGAGAcaaagUUGGCAu-- -3' miRNA: 3'- guGGCCGGU-UCCUUUaa---GACCGUuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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