miRNA: 3'- aUCCugAA----AGGUCucgGUGGGCGUGg -5'
miRNA: 3'- aUCCUGAa-AGGUC----UCGG-------UGGGcgUGG- -5'
miRNA: 3'- auCCUGaaa-GGUCU-CGGUGGG-CGUGG- -5'
miRNA: 3'- auCCugAA---AGGuCUCGG--------UGGGCGUGg -5'
miRNA: 3'- -AUCCUGAAAGGuCUCGGUGGGCGUGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32618 | 5' | -56.2 | NC_007192.1 | + | 2439 | 0.66 | 0.12194 |
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Target: 5'- gGGGugUUguugUCCAGcaaCACCUGCACa -3' miRNA: 3'- aUCCugAA----AGGUCucgGUGGGCGUGg -5' |
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| 32618 | 5' | -56.2 | NC_007192.1 | + | 5730 | 0.67 | 0.099328 |
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Target: 5'- aGGGACUcgUCCAGguauAGCCugguucaACCCuaGCCc -3' miRNA: 3'- aUCCUGAa-AGGUC----UCGG-------UGGGcgUGG- -5' |
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| 32618 | 5' | -56.2 | NC_007192.1 | + | 2526 | 0.67 | 0.090021 |
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Target: 5'- -uGGGCaaagCUGGAuGCCACCCcGUACCu -3' miRNA: 3'- auCCUGaaa-GGUCU-CGGUGGG-CGUGG- -5' |
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| 32618 | 5' | -56.2 | NC_007192.1 | + | 3627 | 0.68 | 0.081269 |
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Target: 5'- -cGGugUUgugUCCuGAGCCaugucgucGCCCGCAUa -3' miRNA: 3'- auCCugAA---AGGuCUCGG--------UGGGCGUGg -5' |
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| 32618 | 5' | -56.2 | NC_007192.1 | + | 1843 | 1.01 | 0.000111 |
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Target: 5'- aUAGGACUUUCC-GAGCCACCCGCACCg -3' miRNA: 3'- -AUCCUGAAAGGuCUCGGUGGGCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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