Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32760 | 3' | -51.1 | NC_007291.1 | + | 7605 | 0.66 | 0.887194 |
Target: 5'- --cCGUGCCAGCugGCuCGUACUUAccACCg -3' miRNA: 3'- cgaGUAUGGUCG--CG-GUAUGAGU--UGGg -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 6638 | 0.66 | 0.854258 |
Target: 5'- -----gGCCuuGCGCCGUACUCuucGCCa -3' miRNA: 3'- cgaguaUGGu-CGCGGUAUGAGu--UGGg -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 42361 | 0.66 | 0.854258 |
Target: 5'- aGCUaCAUcACCAGCGCC----UUGACCUg -3' miRNA: 3'- -CGA-GUA-UGGUCGCGGuaugAGUUGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 24128 | 0.67 | 0.826871 |
Target: 5'- uGCUCGUacucaaGCCAG-GCCAagucCUCuuCCCa -3' miRNA: 3'- -CGAGUA------UGGUCgCGGUau--GAGuuGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 20539 | 0.68 | 0.776927 |
Target: 5'- cUUCAgugACCAGCGCCGguaucuucacUACaUCAaagucgauACCCa -3' miRNA: 3'- cGAGUa--UGGUCGCGGU----------AUG-AGU--------UGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 7150 | 0.68 | 0.755721 |
Target: 5'- uGCUCGUaGCCAuUGCCAaaGCUaaCAACCCu -3' miRNA: 3'- -CGAGUA-UGGUcGCGGUa-UGA--GUUGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 26879 | 0.69 | 0.732865 |
Target: 5'- uGCUUGUGaggucgcUCAGCGCCAUuaaUAACCCa -3' miRNA: 3'- -CGAGUAU-------GGUCGCGGUAugaGUUGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 16891 | 0.7 | 0.654992 |
Target: 5'- aGCUC--ACCAGCaacGCCAgACUUGGCCUu -3' miRNA: 3'- -CGAGuaUGGUCG---CGGUaUGAGUUGGG- -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 8770 | 0.7 | 0.64811 |
Target: 5'- aGCUUGUcGCCAGUGCCAauuaucagucuuauuUGCUCAAUa- -3' miRNA: 3'- -CGAGUA-UGGUCGCGGU---------------AUGAGUUGgg -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 4481 | 0.71 | 0.632036 |
Target: 5'- ---aGUGCCGGCGCgAaACUCAGCUg -3' miRNA: 3'- cgagUAUGGUCGCGgUaUGAGUUGGg -5' |
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32760 | 3' | -51.1 | NC_007291.1 | + | 1744 | 1.14 | 0.001007 |
Target: 5'- uGCUCAUACCAGCGCCAUACUCAACCCc -3' miRNA: 3'- -CGAGUAUGGUCGCGGUAUGAGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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