Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32766 | 3' | -50.8 | NC_007346.1 | + | 373961 | 0.66 | 0.999879 |
Target: 5'- ---uUGCCGUGUUCUuauuuaUGGUGUACAg -3' miRNA: 3'- gcguACGGUAUAAGGca----ACCACGUGU- -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 257693 | 0.67 | 0.999617 |
Target: 5'- cCGCGuuuUGCCAUAUUCCGguaugccGGU-CACc -3' miRNA: 3'- -GCGU---ACGGUAUAAGGCaa-----CCAcGUGu -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 73131 | 0.67 | 0.999617 |
Target: 5'- gGUAUGCCGcAUUaaCUGgaGGUGCAUAu -3' miRNA: 3'- gCGUACGGUaUAA--GGCaaCCACGUGU- -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 78964 | 0.67 | 0.999527 |
Target: 5'- gGCAUG-CAUAUUCCGUU--UGCAUu -3' miRNA: 3'- gCGUACgGUAUAAGGCAAccACGUGu -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 256308 | 0.67 | 0.999527 |
Target: 5'- aCGgGUGCCAac-UCCagUGGUGCGCc -3' miRNA: 3'- -GCgUACGGUauaAGGcaACCACGUGu -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 338307 | 0.68 | 0.99903 |
Target: 5'- aGCAUGCaGUAUUCCGUgcgccagguaacuauUGGaucguUGCGCu -3' miRNA: 3'- gCGUACGgUAUAAGGCA---------------ACC-----ACGUGu -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 74703 | 0.68 | 0.998742 |
Target: 5'- aCGCAUGCacUGUUCCGaucccGG-GCACAa -3' miRNA: 3'- -GCGUACGguAUAAGGCaa---CCaCGUGU- -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 123377 | 0.72 | 0.971651 |
Target: 5'- uGCAauaaaaUGcCCAUAUUCUGUUGGUGgACc -3' miRNA: 3'- gCGU------AC-GGUAUAAGGCAACCACgUGu -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 370783 | 0.72 | 0.971651 |
Target: 5'- uCGCGUGaCCGUccUUCUGUUGGcGCGCGu -3' miRNA: 3'- -GCGUAC-GGUAu-AAGGCAACCaCGUGU- -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 274066 | 0.73 | 0.965739 |
Target: 5'- uGCGU-CCGUAUUUCGacgGGUGCGCGa -3' miRNA: 3'- gCGUAcGGUAUAAGGCaa-CCACGUGU- -5' |
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32766 | 3' | -50.8 | NC_007346.1 | + | 78061 | 1.09 | 0.021326 |
Target: 5'- gCGCAUGCCAUAUUCCGUUGGUGCACAc -3' miRNA: 3'- -GCGUACGGUAUAAGGCAACCACGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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