Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32766 | 5' | -44.3 | NC_007346.1 | + | 140489 | 0.66 | 1 |
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Target: 5'- aUGUucAUACAgUUGGAUGGGggGUGAUUGc -3' miRNA: 3'- -GCA--UAUGUgAGUCUGCUCa-UACUAAC- -5' |
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| 32766 | 5' | -44.3 | NC_007346.1 | + | 248374 | 0.67 | 1 |
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Target: 5'- aGUAUACACUU-GACGAagAUGGUa- -3' miRNA: 3'- gCAUAUGUGAGuCUGCUcaUACUAac -5' |
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| 32766 | 5' | -44.3 | NC_007346.1 | + | 201075 | 0.69 | 1 |
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Target: 5'- gCGgugGUugACUCGGugGAG-AUGGUg- -3' miRNA: 3'- -GCa--UAugUGAGUCugCUCaUACUAac -5' |
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| 32766 | 5' | -44.3 | NC_007346.1 | + | 292167 | 0.67 | 1 |
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Target: 5'- ----cGCGCUCGG-CGGGUAUGGc-- -3' miRNA: 3'- gcauaUGUGAGUCuGCUCAUACUaac -5' |
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| 32766 | 5' | -44.3 | NC_007346.1 | + | 370321 | 0.71 | 0.999998 |
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Target: 5'- aGUAUGCACcaggcaGGGCGAGaUGUGAUa- -3' miRNA: 3'- gCAUAUGUGag----UCUGCUC-AUACUAac -5' |
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| 32766 | 5' | -44.3 | NC_007346.1 | + | 78026 | 1.11 | 0.077649 |
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Target: 5'- uCGUAUACACUCAGACGAGUAUGAUUGg -3' miRNA: 3'- -GCAUAUGUGAGUCUGCUCAUACUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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