Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32767 | 5' | -58.2 | NC_007346.1 | + | 401046 | 0.66 | 0.955535 |
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Target: 5'- uGCCGGGCGUaGAUcGCGUgcgauggaaucaagUCaacuagcuGCCGCGAu -3' miRNA: 3'- -CGGCUCGCA-CUA-CGCG--------------AG--------UGGCGCUu -5' |
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| 32767 | 5' | -58.2 | NC_007346.1 | + | 211939 | 0.67 | 0.940896 |
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Target: 5'- uUCGAGCGcaGUGCGCUCauuuGCCGCu-- -3' miRNA: 3'- cGGCUCGCacUACGCGAG----UGGCGcuu -5' |
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| 32767 | 5' | -58.2 | NC_007346.1 | + | 265978 | 0.67 | 0.936461 |
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Target: 5'- gGCCGAuGUauGUGGUGUGCUCggaaaaacggcGCCGCa-- -3' miRNA: 3'- -CGGCU-CG--CACUACGCGAG-----------UGGCGcuu -5' |
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| 32767 | 5' | -58.2 | NC_007346.1 | + | 364058 | 0.68 | 0.887276 |
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Target: 5'- aGCCGAGCGUucuaauAUGCGUcaUCGCC-CGAu -3' miRNA: 3'- -CGGCUCGCAc-----UACGCG--AGUGGcGCUu -5' |
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| 32767 | 5' | -58.2 | NC_007346.1 | + | 216314 | 0.72 | 0.708979 |
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Target: 5'- cGCCGAGUGUGuccuAUGUGCcgcgCACCGCu-- -3' miRNA: 3'- -CGGCUCGCAC----UACGCGa---GUGGCGcuu -5' |
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| 32767 | 5' | -58.2 | NC_007346.1 | + | 86871 | 1.07 | 0.006392 |
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Target: 5'- gGCCGAGCGUGAUGCGCUCACCGCGAAc -3' miRNA: 3'- -CGGCUCGCACUACGCGAGUGGCGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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